| 龚小雅,宋建宇,吴凤芝.我国不同利用方式土壤四环素类抗性基因积累的研究[J].农业环境科学学报,2023,42(1):87-100. |
| 我国不同利用方式土壤四环素类抗性基因积累的研究 |
| Study on the accumulation of tetracycline resistance genes in soil with different utilization patterns in China |
| 投稿时间：2022-03-11 |
| DOI：10.11654/jaes.2022-0234 |
| 中文关键词: 四环素类抗性基因 设施菜田 露地菜田 露地粮田 |
| 英文关键词: tetracyclines resistance gene facility vegetable field open vegetable field open grain field |
| 基金项目:财政部、农业农村部国家大宗蔬菜产业技术体系项目（CARS-23-B09） |
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| 中文摘要: |
| 为研究我国不同地区的不同利用方式和不同种植年限土壤中抗生素抗性基因绝对丰度的变化，揭示抗性基因在农田土壤中的积累情况，以黑龙江（HLJ）、吉林（JL）、辽宁（LN）、内蒙古（NMG）、新疆（XJ）、甘肃（GS）、云南（YN）、江苏（JS）、湖北（HB）、广东（GD）、海南（HN）、山西（SX）、河南（HEN）、山东（SD）14个省份的土壤为试材，依据土壤利用方式（露地粮田、露地菜田和设施菜田土壤）和种植年限（1~3、7~10 a和15 a以上）设置9个处理，共378个样品。采用普通PCR和荧光定量技术对四环素类抗性基因进行定性和定量分析。四环素类抗生素的4种抗性基因（tetM，tetO，tetQ，tetW）在设施菜田土壤中的绝对丰度显著高于露地粮田和露地菜田，设施菜田绝对丰度范围为3.96×104~6.36×108 copies·g-1，露地粮田为2.16×103~2.18×106 copies·g-1，露地菜田为1.16×104~2.48×107 copies·g-1。4种抗性基因的最高丰度出现在不同种植年限、不同土壤利用方式中的不同省份，但设施菜田3、7 a和15 a的各省份土壤均显著高于相同年限的露地粮田和露地菜田，设施菜田7 a和15 a显著高于3 a。露地粮田、露地菜田的4种抗性基因积累与利用年限无显著线性关系，而设施菜田的抗性基因积累与利用年限存在显著正相关关系。研究表明，四环素类抗性基因已在我国不同地区的粮田、露地菜田和设施菜田土壤中普遍存在，其丰度在设施菜田土壤中显著高于露地菜田和露地粮田土壤，且在设施菜田中与利用年限显著正相关。 |
| 英文摘要: |
| Variations in the absolute abundance of antibiotic resistance genes in different regions of China under different land-use modes and planting years were explored. The purpose of this study is to reveal the accumulation of resistant genes in cropland. Soil from 14 provinces, including Heilongjiang（HLJ）, Jilin（JL）, Liaoning（LN）, Inner Mongolia（NMG）, Xinjiang（XJ）, Gansu（GS）, Yunnan（YN）, Jiangsu（JS）, Hubei（HB）, Guangdong（GD）, Hainan（HN）, Shanxi（SX）, Henan（HEN）, and Shandong（SD）were used in this study. There were nine treatments in total based on different land-use modes（open grain fields, open vegetable fields, and facility vegetable fields）and planting years（1-3, 7-10 years, and more than 15 years）. Tetracycline resistance genes were qualitatively and quantitatively analyzed by polymerase chain reaction（PCR）and quantitative PCR. The absolute abundance of tetracycline resistance genes（tetM, tetO, tetQ, and tetW）in facility soil was significantly higher than that in open grain and vegetable field soil. The absolute abundance in facility vegetable soil was 3.96×104-6.36×108 copies·g-1; in open grain fields, it was 2.16×103-2.18×106 copies·g-1. In open vegetable fields, it was 1.16× 104-2.48 × 107 copies · g-1. The highest absolute abundance of resistance genes appeared in different provinces under different land-use modes and planting years, respectively. However, the greenhouse soil of year 3, 7, and 15 in provinces was significantly higher than that of open grain and vegetable fields for the same years. The facility vegetable soil in years 7 and 15 was significantly higher than in year 3. There was no significant linear relationship between gene accumulation and planting life in open grain and vegetable fields, despite a significantly positive correlation in facility vegetable soil. Tetracycline resistance genes have been ubiquitous in Chinese soil under different land-use modes and planting years. Its abundance was significantly higher in facility vegetable field soil than in open vegetable and open grain field soil. The abundance of facility vegetable fields was significantly positively related to soil planting life. |
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