| 温婧玉,唐鑫,翁靓娴,穆莉,刘潇威,赵玉杰,戴礼洪.黄铁矿和谷氨酸对砷酸盐生菜毒性的调控研究[J].农业环境科学学报,2022,41(10):2120-2130. |
| 黄铁矿和谷氨酸对砷酸盐生菜毒性的调控研究 |
| Implications of pyrite and glutamate in arsenate toxicity in lettuce |
| 投稿时间：2022-02-24 修订日期：2022-04-11 |
| DOI：10.11654/jaes.2022-0175 |
| 中文关键词: 砷酸盐 生菜 黄铁矿 有机物 谷氨酸 |
| 英文关键词: arsenate lettuce pyrite organic matter glutamate |
| 基金项目:国家自然科学基金项目(21876092,22176103);中央科研院所基本科研业务费项目(2022) |
| 作者 | 单位 | E-mail | | 温婧玉 | 农业农村部环境保护科研监测所, 农业农村部农产品质量安全环境因子控制重点实验室, 天津市农业环境与农产品安全重点实 验室, 天津 300191 | | | 唐鑫 | 农业农村部环境保护科研监测所, 农业农村部农产品质量安全环境因子控制重点实验室, 天津市农业环境与农产品安全重点实 验室, 天津 300191 | | | 翁靓娴 | 农业农村部环境保护科研监测所, 农业农村部农产品质量安全环境因子控制重点实验室, 天津市农业环境与农产品安全重点实 验室, 天津 300191 | | | 穆莉 | 农业农村部环境保护科研监测所, 农业农村部农产品质量安全环境因子控制重点实验室, 天津市农业环境与农产品安全重点实 验室, 天津 300191 | muli@caas.cn | | 刘潇威 | 农业农村部环境保护科研监测所, 农业农村部农产品质量安全环境因子控制重点实验室, 天津市农业环境与农产品安全重点实 验室, 天津 300191 | | | 赵玉杰 | 农业农村部环境保护科研监测所, 农业农村部农产品质量安全环境因子控制重点实验室, 天津市农业环境与农产品安全重点实 验室, 天津 300191 | | | 戴礼洪 | 农业农村部环境保护科研监测所, 农业农村部农产品质量安全环境因子控制重点实验室, 天津市农业环境与农产品安全重点实 验室, 天津 300191 | |
|
| 摘要点击次数: 669 |
| 全文下载次数: 736 |
| 中文摘要: |
| 为探究含铁矿物和有机物添加对生菜砷酸盐毒性的调控作用，采用高分辨扫描电镜和X射线光电子能谱表征培养液中外源添加物与砷（As）之间的相互作用，采用高效液相色谱串联电感耦合等离子体质谱法测定不同处理组生菜中As的存在形态及累积变化状况，并综合考察了营养元素含量、超氧化物酶浓度和丙二醛含量等抗氧化系统和纤维素等指标的变化。结果表明：培养液中的As通过物理吸附或化学作用力结合在黄铁矿表面，添加谷氨酸促进了该过程的发生，从而抑制了As从植物根部向可食部的迁移。当生菜生长至第20~40天，相比黄铁矿单独处理，黄铁矿和谷氨酸组合处理使无机As浓度降低35.9%，总As浓度降低16.9%；与谷氨酸单独处理相比，无机As浓度降低4.0%，总As浓度降低21.8%。在植物叶片中，相比As （Ⅴ）单独处理组，黄铁矿和谷氨酸的添加使叶质量和叶绿素含量分别提高了20.8%~30.8%和17.1%~25.5%，通过增加叶片中超氧化物酶的合成限制活性氧在体内的积累，减少脂质过氧化反应。黄铁矿添加具有促进营养元素吸收的作用，而谷氨酸添加有利于缓解由As胁迫引起的植物氧化应激作用。相比黄铁矿单独处理，黄铁矿和谷氨酸组合处理更有利于植物对镁（Mg）、锰（Mn）、铜（Cu）和铁（Fe）元素的吸收，增强植物对As的抗逆性。同时，与谷氨酸或黄铁矿单独处理相比，二者组合处理提升了植物的品质，分别使维生素C含量提高了42.5%和69.3%，纤维素含量提高了21.2%和374.6%，蛋白质含量提高了71.4%和21.4%。研究表明，黄铁矿和谷氨酸组合处理能有效降低溶液中As的迁移和生菜叶片中As的积累，有利于植物体中活性氧的清除，并可提高植物对营养元素的吸收，促进品质改善。 |
| 英文摘要: |
| This study was aimed to evaluate the role of iron-containing minerals and organic matter in the regulation of arsenate toxicity in lettuce. High-resolution scanning electron microscopy and X-ray photoelectron spectroscopy were used to characterize the interaction between exogenous additives and arsenic in a Hoagland solution, and high-performance liquid chromatography coupled with inductively coupled plasma mass spectrometry（HPLC-ICP-MS）was used to determine the morphological and cumulative changes in arsenic in lettuce in different treatment groups and investigate the changes in nutrient content, superoxide dismutase concentration, and malondialdehyde concentration in the antioxidant system and fiber. Results showed that arsenic in the culture solution was physically adsorbed or chemically bound to the surface of pyrite, and glutamate addition facilitated this process, thus hindering the migration of arsenic from the roots to the edible parts of the plants. When lettuce was grown for 20-40 days, the combined treatment of pyrite and glutamate reduced the inorganic arsenic and total arsenic concentrations by 35.9% and 16.9%, respectively, compared to that by pyrite treatment alone and by 4.0% and 21.8%, respectively, compared to that by glutamic acid treatment alone. In leaves, pyrite and glutamate addition increased leaf weight by 20.8% - 30.8% and chlorophyll content by 17.1% - 25.5% compared to that by the addition of pentavalent arsenic alone, limiting the accumulation of reactive oxygen species in the body by increasing the synthesis of superoxide dismutase in the leaves and reducing lipid peroxidation reactions. Pyrite addition promoted nutrient uptake, whereas glutamate addition was beneficial in alleviating oxidative stress in plants caused by arsenic stress. Compared to pyrite treatment alone, the combined pyrite and glutamate treatment was more beneficial to the uptake of Mg, Mn, Cu, and Fe elemental nutrients by plants and enhanced plant resistance to arsenic. In addition, the combined treatment enhanced the quality of the plants compared to glutamate or pyrite treatment alone, increasing the vitamin C content by 42.5% and 69.3%, cellulose content by 21.2% and 374.6%, and protein content by 71.4% and 21.4%, respectively. The combined treatment effectively reduced the migration of arsenic in solution and accumulation of arsenic in lettuce leaves, which consequently facilitated the removal of reactive oxygen species from plants, increased the uptake of nutrient elements, and improved the quality of plants. |
| HTML
查看全文
查看/发表评论 下载PDF阅读器 |
|
|
|
