| 买迪努尔·阿不来孜,陈末,杨再磊,愚广灵,胡洋,贾宏涛.巴音布鲁克高寒湿地土壤真菌群落对不同程度退化的响应[J].农业环境科学学报,2022,41(8):1778-1787. |
| 巴音布鲁克高寒湿地土壤真菌群落对不同程度退化的响应 |
| Responses of soil fungal communities to different degrees of degradation in Bayinbuluk alpine wetland, China |
| 投稿时间：2021-11-29 |
| DOI：10.11654/jaes.2021-1383 |
| 中文关键词: 高寒湿地 退化 真菌 群落多样性 群落结构 |
| 英文关键词: alpine wetland degradation fungal community diversity community composition |
| 基金项目:国家自然科学基金项目（31560171）；新疆维吾尔自治区研究生创新项目（XJ2021G167） |
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| 中文摘要: |
| 为了解巴音布鲁克高寒湿地土壤真菌群落对退化的响应，探索湿地退化机制，本研究以巴音布鲁克高寒湿地未退化（ND）、轻度退化（SD）及重度退化（HD）区域的土壤为研究对象，采用高通量测序技术分析不同程度退化高寒湿地土壤真菌群落的多样性及结构差异，并结合理化指标进一步分析影响真菌群落的环境因子。结果表明：真菌群落Simpson和Shannon多样性指数在ND和HD区土壤中无显著差异（P>0.05）；SD区土壤中的真菌群落Simpson指数显著高于ND和HD区，而Shannon指数显著低于ND和HD区（P<0.05）。在不同程度退化区土壤中，真菌群落ACE和Chao1丰富度指数均随退化的加剧呈降低趋势。在3个区域土壤中，门水平上，子囊菌门（Ascomycota）的相对丰度最高；纲水平上，粪壳菌纲（Sordariomycetes）为主导类群；肉座菌目（Hypocreales）、Pleosporales菌目、被孢霉目（Mortierellales）的相对丰度均随退化程度的增加而增加；属水平上，珊瑚菌属（Clavaria）、稻瘟病菌属（Magnaporthe）为优势菌属。LEfSe分析发现了11种可作为判断退化的潜在生物标志物。RDA分析及蒙特卡罗检验结果显示，在门水平上真菌群落结构变化不受环境因子的显著影响；在属水平上，真菌群落结构变化仅与土壤总有机碳存在显著相关性（P<0.05）。研究表明，高寒湿地退化显著改变了土壤pH、土壤含水量、总有机碳、容重等理化指标，进而影响真菌群落多样性和结构。 |
| 英文摘要: |
| Exploring the response of soil fungal community to degradation in Bayinbuluk alpine wetland is of great significance to the study of wetland degradation mechanisms. In this research, the non-degraded(ND), slightly degraded(SD), and severely degraded(HD)regions of Bayinbuluk alpine wetland were used as the research objects. The diversity and structural differences of soil fungal communities in differently degraded alpine wetlands were analyzed by high-throughput sequencing technology, and the environmental factors affecting fungal communities were further analyzed in combination with physicochemical indexes. The results showed that there was no significant difference in Simpson and Shannon diversity indexes between the ND and HD areas(P>0.05). However, the Simpson index in the SD area were significantly higher than that in the ND and HD areas, while the Shannon index were significantly lower than in the ND and HD areas (P<0.05). ACE and Chao1 richness indexes of fungal communities decreased with increasing degradation. At phylum level, the relative abundance of Ascomycota was the highest, whereas Sordariomycetes showed the highest relative abundance in three regions at the class level. The relative abundance of the orders Hypocreales, Pleosporales, and Mortierellales increased with the degree of degradation. At the genus level, Clavaria and Magnaporthe were the dominant fungal communities. LEfSe analysis found 11 potential biomarkers that could be used as indicators of degradation. Redundancy analysis and Monte Carlo test showed that the changes in fungal community structure at the phylum level were not significantly affected by environmental factors(P<0.05); At the genus level, changes in fungal community structure are only significantly affected by total organic carbon(P<0.05). The degradation of alpine wetland significantly changed soil pH, soil moisture content, total organic carbon, bulk density, and other physicochemical indexes, affecting the diversity and structure of fungal communities. |
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