| 杨昳,陈元晖,张春燕,张羽,李明堂.复合菌和鸡粪生物炭对镍和镉污染土壤的修复效果研究[J].农业环境科学学报,2022,41(8):1709-1719. |
| 复合菌和鸡粪生物炭对镍和镉污染土壤的修复效果研究 |
| Remediation effect of compound bacteria and chicken manure-derived biochar in nickel- and cadmium-contaminated soil |
| 投稿时间：2021-12-27 |
| DOI：10.11654/jaes.2021-1491 |
| 中文关键词: 镍 镉 土壤修复 多功能微生物 生物炭 微生物群落 |
| 英文关键词: nickel cadmium soil remediation multifunctional microorganism biochar microbial community |
| 基金项目:吉林省重点研发计划项目（20200403003SF）；国家自然科学基金项目（42077137） |
|
| 摘要点击次数: 849 |
| 全文下载次数: 834 |
| 中文摘要: |
| 微生物和生物炭通过协同作用可实现重金属污染土壤的低碳、绿色修复，但是目前相关研究较少。本研究通过玉米盆栽试验，研究了多功能复合菌和鸡粪生物炭对镍（Ni）和镉（Cd）污染土壤的修复。研究结果表明，单一和共同作用下复合菌和不同粒径生物炭均能不同程度地固定土壤中的Ni和Cd，减轻Ni和Cd的毒害并降低玉米幼苗对Ni和Cd的吸收，增加根际土壤细菌、真菌群落结构丰富度与物种多样性，其中复合菌和粒径较小的微米生物炭（1.6~55.8 μm）联合修复效果最好。与对照相比，复合菌和微米生物炭联合修复下根际土壤中碳酸盐结合态Ni和Cd的含量分别升高了59.9%和68.4%，有效态Ni和Cd的含量降低了66.5%和53.8%，促进了盆栽玉米幼苗的生长发育，提高了抗氧化能力，使玉米根部的Ni和Cd含量分别降低了49.3%和41.9%，地上部的Ni和Cd含量分别降低了69.4%和53.0%，降低了Ni和Cd在叶片细胞器中的占比，并以低毒形态存在；根际土壤中细菌和真菌的OTU数目显著增加，优势菌群的丰富度和多样性提高，根际土壤微生物群落结构改善。以上研究结果表明多功能微生物和生物炭具有协同修复效应，可为重金属污染土壤联合修复提供理论依据和技术支持。 |
| 英文摘要: |
| Microorganisms and biochar may achieve synergistic low-carbon, green remediation of heavy metal-contaminated soils, but there has been little research on this topic. In the present study, a corn pot experiment was performed to evaluate the remediation of nickel (Ni)- and cadmium(Cd)-contaminated soil by multifunctional compound bacteria and chicken manure-derived biochar. In both single and combined remediation experiments, compound bacteria and biochar with different particle sizes immobilized Ni and Cd in the soil to varying degrees, reduced the uptake of Ni and Cd in maize seedlings, and increased the structural richness and species diversity of bacteria and fungi in rhizosphere soil. The most efficient remediation effect was attributed to the combination of compound bacteria and micronscale biochar with a smaller particle size(1.6~55.8 μm). Using this remediation strategy, the amount of available Ni and Cd decreased by 66.5% and 53.8%, respectively, and the amount of carbonate-bound Ni and Cd increased by 59.9% and 68.4%, respectively, in rhizosphere soil. Moreover, combined remediation with compound bacteria and micron-scale biochar resulted in improved growth performance and increased antioxidant capacity of potted maize seedlings. The Ni and Cd content in maize roots decreased by 49.3% and 41.9%, respectively, and the Ni and Cd content of the aboveground parts of the plants decreased by 69.4% and 53.0%, respectively, after combined remediation. Combined remediation with compound bacteria and micron-scale biochar also increased biological activity in rhizosphere soil, as indicated by an increased number of operational taxonomic units of bacteria and fungi, improved richness and diversity of the dominant microbial flora, and improved microbial community structure. These results show that multifunctional microorganisms and biochar have synergistic remediation effect, and provide the theoretical basis and technical support for the combined remediation of heavy metal-contaminated soil. |
| HTML
查看全文
查看/发表评论 下载PDF阅读器 |
|
|
|
