| 林钰栅,刘景春,卢豪良,丁友芳,严重玲.红树根际解磷真菌筛选及其对桐花树生长的影响[J].农业环境科学学报,2022,41(5):950-958. |
| 红树根际解磷真菌筛选及其对桐花树生长的影响 |
| Screening phosphate-solubilizing fungi from the mangrove rhizosphere and their effect on Aegiceras corniculatum seedling growth |
| 投稿时间：2021-11-27 |
| DOI：10.11654/jaes.2021-1376 |
| 中文关键词: 解磷真菌 根际 桐花树 促生作用 |
| 英文关键词: phosphate-solubilizing fungi rhizosphere Aegiceras corniculatum growth-promoting effect |
| 基金项目:国家自然科学基金项目（31870483，31530008） |
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| 中文摘要: |
| 为了获取具有解磷、促生作用的红树根际解磷真菌，采用NBRIP（National Botanical Research Institute's phosphate）培养基初筛法从红树根际土壤筛选得到解磷真菌，并分析菌株对不同难溶性磷酸盐的溶解差异和高效解磷真菌对桐花树的促生效果。结果表明：解磷能力最强的PSF-FJ1、PSF-FJ2和PSF-FJ3菌株对该生境中具有代表性的难溶性磷酸盐的溶解顺序为Ca3（PO4）2>AlPO4>FePO4>卵磷脂，菌株PSF-FJ1对Ca3（PO4）2的溶解能力可达773.36 mg·L-1，培养液酸化是溶解Ca3（PO4）2的关键机制之一。通过ITS基因序列分析方法，将具有吲哚-3-乙酸（IAA）和铁载体合成潜力的高效解磷真菌PSF-FJ1鉴定为Penicillium sp.。与未接菌相比，接种PSF-FJ1使得桐花树幼苗的总生物量提高了23.13%，植株根系活力增强了64.91%，叶片Pn、Tr和Gs分别显著提高37.92%、40.04%和41.70%，叶片光合色素含量显著增加，更多的磷元素和氮元素被植株吸收。研究表明，解磷真菌Penicillium sp.PSF-FJ1可有效促进桐花树幼苗的生长，该菌株可开发为新型生物菌肥并可尝试应用于滨海红树造林工程。 |
| 英文摘要: |
| To obtain phosphate-solubilizing fungi（PSF）with the function of P-solubilization and as a plant growth promoter, the phosphate medium of the National Botanical Research Institute was employed to screen PSF strains from mangrove rhizospheric soils. The P-solubilizing capacity of multiple insoluble phosphates of PSF strains and their growth-promoting effect on Aegiceras corniculatum were further evaluated. The results showed that three fungi strains（PSF-FJ1, PSF-FJ2, and PSF-FJ3）were able to solubilize various insoluble phosphates in the order Ca3（PO4）2 >AlPO4 >FePO4 > lecithin. The PSF-FJ1 strain exhibited the strongest Ca3（PO4）2-solubilizing activity （773.36 mg·L-1）, and medium acidification was considered the key principal P-solubilization mechanism with Ca3（PO4）2. The PSF-FJ1 strain was identified as being from the genus Penicillium based on ITS sequence analysis, which included indole-3-acetic acid and siderophores synthetic capacity. Relative to the control, the inoculation of Penicillium sp. PSF-FJ1 strain increased total biomass by 23.13%; enhanced root vitality by 64.91%; improved Pn, Tr, and Gs by 37.92%, 40.04%, and 41.70%, respectively; and increased the leaf photosynthetic pigment content and P and N uptake. Therefore, as the application of the Penicillium sp. PSF-FJ1 strain positively improved the growth of A. corniculatum seedlings, it has potential for use as a biofertilizer in the reforestation of coastal mangrove wetlands. |
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