| 刘凯,陈修报,刘洪波,姜涛,杨健.背角无齿蚌幼蚌不同组织的铜积累特征[J].农业环境科学学报,2022,41(5):943-949. |
| 背角无齿蚌幼蚌不同组织的铜积累特征 |
| Copper accumulation patterns in different tissues of juvenile Anodonta woodiana individuals |
| 投稿时间：2021-09-01 |
| DOI：10.11654/jaes.2021-1002 |
| 中文关键词: 背角无齿蚌 铜 生物积累 靶组织 暴露时间 暴露浓度 暴露浓度 |
| 英文关键词: Anodonta woodiana copper bioaccumulation target tissue exposure time exposure concentration exposure concentration |
| 基金项目:中国水产科学研究院基本科研业务费（2019GH10）；中国水产科学研究院科技创新团队项目（2020TD18）；国家自然科学基金青年科学基金项目（31502166） |
| 作者 | 单位 | E-mail | | 刘凯 | 南京农业大学无锡渔业学院, 江苏 无锡 214081 | | | 陈修报 | 中国水产科学研究院淡水渔业研究中心, 中国水产科学研究院长江中下游渔业生态环境评价和资源养护重点实验室, 江苏 无锡 214081 | | | 刘洪波 | 中国水产科学研究院淡水渔业研究中心, 中国水产科学研究院长江中下游渔业生态环境评价和资源养护重点实验室, 江苏 无锡 214081 | | | 姜涛 | 中国水产科学研究院淡水渔业研究中心, 中国水产科学研究院长江中下游渔业生态环境评价和资源养护重点实验室, 江苏 无锡 214081 | | | 杨健 | 南京农业大学无锡渔业学院, 江苏 无锡 214081
中国水产科学研究院淡水渔业研究中心, 中国水产科学研究院长江中下游渔业生态环境评价和资源养护重点实验室, 江苏 无锡 214081 | jiany@ffrc.cn |
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| 中文摘要: |
| 为探究铜（Cu）在“淡水贝类观察”研究体系指示生物——背角无齿蚌（Anodonta woodiana）中的积累特征，本研究基于对重金属毒性更为敏感的幼蚌，根据Cu对幼蚌96 h-EC50和我国《渔业水质标准》（GB 11607—1989）中Cu限量设定5个浓度（2.0、1.0、0.1、0.01、0.005 mg·L-1），进行了24、48、72、96 h暴露及组织（鳃、外套膜、斧足、消化腺、其余组织及整个软组织）Cu含量的ICP-MS测定。结果显示：暴露组各组织中Cu含量均显著高于对照组（P<0.05）；鳃对Cu积累反应最为灵敏，在暴露24 h时Cu含量就显著增加（P<0.05），且显著高于斧足和消化腺中Cu含量（P<0.05）；外套膜在暴露72 h后Cu积累量最高，含量（干质量）可达到669.2μg·g-1；整个软组织中的Cu含量随暴露浓度的升高和暴露时间的延长呈增加趋势。研究结果显示，背角无齿蚌幼蚌的鳃和外套膜是水体Cu积累的潜在靶组织。 |
| 英文摘要: |
| This study aimed to investigate the tissue-specific toxic effects of copper（Cu）on the freshwater mussel Anodonta woodiana at the juvenile stage, when this species is more sensitive to heavy metal toxicity. Five different Cu concentrations（2.0, 1.0, 0.1, 0.01 mg·L-1, and 0.005 mg·L-1）were set, based on the results of a preliminary 96 h-EC50 experiment and the China Water Quality Standard for Fisheries （GB 11607—1989）. The mussels were exposed to Cu for 24, 48, 72 h, and 96 h, and tissue Cu content were assayed by inductively coupled plasma mass spectrometry（ICP-MS）. Such analysis showed that Cu content in the exposed groups were significantly higher than those in the control groups. In particular, Cu content in the whole soft tissue increased with increasing exposure Cu concentrations and longer exposure times. The highest tissue-specific Cu content was observed in the mantle, with a value of 669.2 μg·g-1. The gill and mantle were the most sensitive tissues, since they displayed significantly increased Cu content already after 24 h of exposure, and reached Cu levels that were significantly higher than those in the foot and digestive glands. Cu content in gill and mantle tissues reached a maximum at 96 h and 72 h, respectively. Nevertheless, the content of Cu at 96 h was highest in the digestive glands and axe-shaped foot. Therefore, these tissues are potential targets for Cu accumulation. This study provides a theoretical basis for the characterization of Cu accumulation in tissues of A. woodiana, the bioindicator species of the“Freshwater Mussel Watch”project, as well as technical support for monitoring and evaluating Cu pollution in freshwater environments. |
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