| 刘玲,洪婷婷,胡倩男,谢瑞丽,周颖,王玲,汪承润.微塑料与铅复合污染对水稻幼苗根系生长和氧化应激的影响[J].农业环境科学学报,2021,40(12):2623-2633. |
| 微塑料与铅复合污染对水稻幼苗根系生长和氧化应激的影响 |
| Effects of the combination of microplastics and lead pollution on growth and oxidative responses of rice seedlings' roots |
| 投稿时间：2021-05-05 |
| DOI：10.11654/jaes.2021-0523 |
| 中文关键词: 水稻 聚苯乙烯微塑料 铅 氧化应激 协同效应 |
| 英文关键词: rice polystyrene microplastics lead oxidative response synergistic effect |
| 基金项目:安徽省重大专项项目（18030701189） |
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| 中文摘要: |
| 为探讨聚苯乙烯微塑料（PS-MPs）、铅（Pb）及其复合污染对水稻幼苗根系生长和氧化应激的影响，研究了水稻幼苗分别暴露于10~40 mg·L-1 PS-MPs、20 μmol·L-1 Pb及其复合溶液后根长、根系生物量、氧化损伤、抗氧化酶活性和Pb含量的变化。结果表明，与空白对照比较，PS-MPs处理降低了水稻幼苗的鲜质量、干质量和根长，显著诱导了丙二醛（MDA）和超氧自由基（O2-·）的产生以及根尖细胞坏死，还不同程度提高了根部超氧化物歧化酶（SOD）和抗坏血酸过氧化物酶（APX）活性。与Pb单一处理比较，10~20 mg·L-1 PS-MPs+Pb减少了根部对Pb的吸收累积，增加了根长和鲜质量，降低了MDA和O2-·产物，减轻了根尖细胞的氧化损伤，而40 mg·L-1 PS-MPs+Pb则增加了Pb含量，降低了根长和鲜质量，促进了MDA和O2-·的积累，并加剧了根尖细胞的坏死。另外，高浓度PS-MPs+Pb还降低了SOD和过氧化氢酶（CAT）活性，同时提高了愈创木酚过氧化物酶（POD）活性。研究表明，PSMPs诱导了水稻幼苗根系的氧化损伤并抑制其生长；低浓度PS-MPs能够缓解Pb对水稻幼苗根系的氧化胁迫，而高浓度PS-MPs则可能与Pb产生了协同作用，加剧了Pb对水稻根系的氧化损伤。 |
| 英文摘要: |
| In order to investigate the effect of polystyrene microplastics(PS-MPs), lead(Pb), and their combination on the growth and oxidative responses of rice seedling roots, changes in root lengths, growth, oxidative damage, antioxidant enzyme activities, and Pb concentrations were examined in the roots of rice seedlings hydroponically cultured in 10~40 mg·L-1 PS-MPs with 20 μmol·L-1 Pb, and their combined solutions. The results showed that, in comparison to the control, the fresh weight, dry weight, and root length were decreased, whereas the products of malondialdehyde(MDA) and superoxide free radicals(O2-·) and root tip necrosis were significantly increased under cultivation with PS-MPs. Meanwhile, the activities of superoxide dismutase(SOD) and ascorbate peroxidase(APX) were differentially elevated in the roots following PS-MP treatment. Compared with Pb treatment alone, treatment with 10~20 mg·L-1 PS-MPs+ Pb resulted in a reduction of Pb accumulation, an increase in root length and fresh weight, and decrease in MDA and O2-·products and alleviation of oxidative damage in the roots. However, 40 mg·L-1 PS-MPs+Pb improved the Pb concentration, diminished the root length and fresh weight, and elevated MDA and O2-· products and aggravated necrosis in the roots. Moreover, higher concentrations of PS-MPs, in combination with Pb, also led to a decline in SOD and catalase activities but enhanced the guaiacol peroxidase activity in the roots. These results indicate that the tested PS-MPs cause oxidative stress and inhibite the growth of the rice seedling roots. PS-MPs at lower concentrations alleviate the Pb-induced oxidative stress, whereas at higher concentrations, it might produce a synergistic effect with Pb, thus aggravating the oxidative damage caused by Pb exposure in the roots of rice seedlings. |
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