| 王北南,宋晓,贺琳娟,钱永忠,邱静.莠去津和芴对斑马鱼胚胎的联合毒性效应研究[J].农业环境科学学报,2021,40(10):2086-2094. |
| 莠去津和芴对斑马鱼胚胎的联合毒性效应研究 |
| Joint toxicity of atrazine and fluorene on zebrafish embryos |
| 投稿时间：2021-03-04 |
| DOI：10.11654/jaes.2021-0261 |
| 中文关键词: 莠去津 芴 斑马鱼 氨基酸代谢组学 联合毒性 |
| 英文关键词: atrazine fluorene zebrafish amino acid metabolomics joint toxicity |
| 基金项目:国家重点研发计划项目（2017YFC1600705） |
| 作者 | 单位 | E-mail | | 王北南 | 中国农业科学院农业质量标准与检测技术研究所, 农业农村部农产品质量安全重点实验室, 北京 100081 | | | 宋晓 | 中国农业科学院农业质量标准与检测技术研究所, 农业农村部农产品质量安全重点实验室, 北京 100081 | | | 贺琳娟 | 中国农业科学院农业质量标准与检测技术研究所, 农业农村部农产品质量安全重点实验室, 北京 100081 | | | 钱永忠 | 中国农业科学院农业质量标准与检测技术研究所, 农业农村部农产品质量安全重点实验室, 北京 100081 | | | 邱静 | 中国农业科学院农业质量标准与检测技术研究所, 农业农村部农产品质量安全重点实验室, 北京 100081 | qiujing@caas.cn |
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| 中文摘要: |
| 为了研究莠去津和芴的联合毒性效应，以斑马鱼作为模式生物，进行胚胎发育暴露实验、联合毒性模型预测和靶向氨基酸代谢组学研究。结果表明，在联合暴露120 h后的斑马鱼仔鱼中均观察到异常发育情况，会引起斑马鱼胚胎卵黄囊异常和脊柱弯曲，其毒性作用方式分别为拮抗作用和协同作用，且无论低高剂量下，二元联合暴露比单一暴露时毒性风险更高。靶向代谢组学研究发现，污染物主要通过干扰甘氨酸、丝氨酸、苏氨酸和组氨酸代谢等途径影响斑马鱼仔鱼的氨基酸代谢。研究表明，莠去津和芴的联合暴露对斑马鱼胚胎的神经发育、氧化应激、抗炎机制、能量代谢、免疫和细胞凋亡机制等生理功能可能产生影响，进而影响其生长发育，且卵黄囊异常是较为敏感的毒性指标。 |
| 英文摘要: |
| To study the combined toxicity effects of atrazine and fluorene, zebrafishes were used as model organisms to carry out embryo development exposure experiments, combined toxicity model prediction, and targeted amino acid metabolomics research. The results showed that abnormal development was observed in zebrafish larvae after 120 h of joint exposure, leading to abnormal embryo yolk sac and curvature of the spine. The toxic action modes exhibited antagonistic and synergistic effects. Regardless of doses, the dual combined exposure had a higher risk of toxicity than single exposure. Targeted metabolomic studies found that pollutants mainly affect the amino acid metabolism of zebrafish larvae by interfering with the metabolism of glycine, serine, threonine, and histidine. The study showed that the combined exposure of atrazine and fluorene might interfere with physiological functions of zebrafish embryos, such as neural development, oxidative stress, anti-inflammatory mechanisms, energy metabolism, and immunity and apoptosis mechanisms, which in turn affects growth and development, with abnormal yolk sac being an indicator of toxicity. |
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