| 朱文博,张秋萍,许继飞,庞小可,刘建国,赵吉.牛粪原料浓度对厌氧消化削减四环素类抗性基因的影响[J].农业环境科学学报,2021,40(8):1808-1817. |
| 牛粪原料浓度对厌氧消化削减四环素类抗性基因的影响 |
| Role of substrate concentrations in the removal of tetracycline resistance genes in anaerobic digestion of dairy manure |
| 投稿时间：2021-01-11 |
| DOI：10.11654/jaes.2021-0033 |
| 中文关键词: 厌氧消化 原料浓度 牛粪 四环素类抗性基因 微生物群落 |
| 英文关键词: anaerobic digestion substrate concentration dairy manure tetracycline resistance genes(TRGs) microbial communities |
| 基金项目:国家自然科学基金项目（51768048）；内蒙古自然科学基金项目（2020MS05003）；内蒙古自治区科技计划项目（2020GG0015，2020GG0081） |
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| 中文摘要: |
| 为了探究不同原料浓度厌氧消化体系中四环素类抗性基因（Tetracycline resistance genes，TRGs）的变化，设置牛粪原料浓度为100%、75%、50%和25%，分析了TRGs、理化因子和微生物群落的变化特征及三者之间的相互关系。结果表明：不同处理之间产气速率相似，原料浓度从100%降低至25%时总产气量从358.83 mL·g-1·d-1增加至400.89 mL·g-1·d-1。消化后的大部分处理中tetO、tetQ和tetT相对丰度减小为原料的1.04%~87.06%，而tetC、tetG、tetW和tetX相对丰度增加至原料的1.32~34.99倍。与100%原料浓度相比，较低原料浓度消化后多数TRGs如tetC、tetG、tetO和tetX以及intⅠ1的相对丰度更高。不同条件下微生物群落差异明显，较低浓度原料（50%和25%）消化后优势菌门从Firmicutes变为Proteobacteria，且TRGs潜在宿主菌属的种类和数目均随着原料浓度的改变而改变。冗余分析表明，理化因子通过影响潜在宿主菌的变化从而影响TRGs的变化。综上，由废水干预的牛粪原料浓度较低的情况可能有利于TRGs潜在宿主菌的增殖，从而增加TRGs传播的风险。 |
| 英文摘要: |
| This study investigated the changes in tetracycline resistance genes(TRGs) in thermophilic anaerobic digestion with different substrate concentrations of 100%, 75%, 50%, and 25%, and the changes in TRGs, physical and chemical factors, and microbial communities and their relationships were analyzed. The results showed that the biogas production rates were similar among four treatments, and the total biogas yields increased from 358.83 mL·g-1·d-1 to 400.89 mL·g-1·d-1 as substrate concentration decreased from 100% to 25%. After anaerobic digestion, the relative abundances of tetO, tetQ, and tetT decreased by 1.04%~87.06%, whereas those of tetC, tetG, tetW, and tetX increased by 1.32~34.99 times. Compared with 100% substrate concentration, relatively lower substrate concentrations had the higher relative abundances of target genes after anaerobic digestion, such as tetC, tetG, tetO, tetX, and intⅠ1. Differences in microbial communities were observed among the four treatments. The dominant phylum changed from Firmicutes to Proteobacteria after digestion with lower substrate concentrations(50% and 25%). The species and number of potential host bacteria of TRGs also altered with the changes in substrate concentrations. Redundancy analysis demonstrated that physicochemical parameters affected the changes in TRGs by affecting their potential host bacteria. In conclusion, a low concentration of substrate due to the intervention wastewater might benefit the proliferation of potential host bacteria, thereby increasing the risks of transmission of TRGs. |
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