| 吴桐,路强强,赵叶子,陈智坤,刑国强,贾锐鱼.基于木质素单体含量的园林生物质降解菌评价[J].农业环境科学学报,2021,40(7):1575-1583. |
| 基于木质素单体含量的园林生物质降解菌评价 |
| Evaluation of garden biomass-degrading bacteria based on lignin monomer content |
| 投稿时间：2020-12-23 |
| DOI：10.11654/jaes.2020-1480 |
| 中文关键词: 园林生物质 木质素单体 降解菌株 酶活力 降解评价 |
| 英文关键词: garden biomass lignin monomer degradation strain enzyme activity degradation evaluation |
| 基金项目:陕西省科学院科技计划项目（2018K-11，2020K-08）；西安市科技计划项目（2193058YF046NS046） |
| 作者 | 单位 | E-mail | | 吴桐 | 陕西省科学院土壤资源与生物技术应用重点实验室, 陕西省西安植物园(陕西省植物研究所), 西安 710061
西安科技大学地质与环境学院, 西安 710043 | | | 路强强 | 陕西省科学院土壤资源与生物技术应用重点实验室, 陕西省西安植物园(陕西省植物研究所), 西安 710061 | luqiang@xab.ac.cn | | 赵叶子 | 陕西省科学院土壤资源与生物技术应用重点实验室, 陕西省西安植物园(陕西省植物研究所), 西安 710061
西安科技大学地质与环境学院, 西安 710043 | | | 陈智坤 | 陕西省科学院土壤资源与生物技术应用重点实验室, 陕西省西安植物园(陕西省植物研究所), 西安 710061 | | | 刑国强 | 西安市阎良区国强瓜菜专业合作社, 西安 710089 | | | 贾锐鱼 | 西安科技大学地质与环境学院, 西安 710043 | |
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| 中文摘要: |
| 基于降解剩余物中木质素单体含量变化，对园林生物质降解菌的酶解效果进行评价。以羧甲基纤维素钠为唯一碳源，对天然林下腐植质层降解菌进行功能筛选和分子鉴定；通过正交实验优化酶活力、降解试验分析降解失重率，经气相色谱-质谱联用技术测定降解剩余物中木质素单体含量，并综合评价降解效率。结果表明：酶活力较高的株菌QL-1、QL-4分别为曼氏假单胞菌（Pseudomonas mandelii）和烟曲霉（Aspergillus fumigatus），其纤维素酶活力分别为14.10 U·mL-1和8.15 U·mL-1；培养10 d时两株菌的降解失重率达到最高，分别为9.48%和13.91%，是未添加降解菌株即空白对照组的2.49倍和3.66倍；愈创木基和紫丁香基木质素单体含量均有显著减少，10 d时QL-4试验组的净降解率为15.57%，是QL-1的3.15倍。研究表明，筛选所得烟曲霉具更强降解活力，其降解剩余物中木质素单体含量均有不同程度下降。 |
| 英文摘要: |
| Based on the change in the lignin monomer content in the degradation residue, the enzymatic hydrolysis effect of garden biomass-degrading bacteria was evaluated. Using sodium carboxymethyl cellulose as the sole carbon source, functional screening and molecular identification of natural understory humus layer-degrading bacteria were conducted. The enzyme activity was optimized through orthogonal experiments and a degradation test was used to analyze the degradation weight loss rate. Gas chromatography-mass spectrometry was used to determine the lignin monomer content in the degradation residue and comprehensively evaluate the degradation efficiency. Strains QL-1 and QL-4, which had a higher enzyme activity, were Pseudomonas mandelii and Aspergillus fumigatus, respectively, and their cellulase activities were 14.10 U·mL-1 and 8.15 U·mL-1, respectively. The degradation rates of the two strains reached the highest levels of 9.48% and 13.91%, respectively, when they were cultured for 10 days, which were 2.49 times and 3.66 times greater than that of the blank control group, respectively. There was a significant reduction in the lignin monomer contents of the guaiacyl and syringyl units. The net degradation rate of the QL-4 test group was 15.57% at 10 days, which was 3.15 times greater than that of QL-1. The screened Aspergillus fumigatus had a stronger degradation activity, and the lignin monomer content in the degradation residues decreased to different degrees. |
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