| 吕耀,王立立,徐智敏,李取生,林欣,周婷,叶汉杰,高琼.不同有机酸对土壤杆菌在菜心根际定殖的影响[J].农业环境科学学报,2018,37(9):1918-1924. |
| 不同有机酸对土壤杆菌在菜心根际定殖的影响 |
| Effect of various organic acids on Agrobacterium species colonization in the rhizosphere of Chinese flowering cabbages |
| 投稿时间：2018-02-05 |
| DOI：10.11654/jaes.2018-0198 |
| 中文关键词: 土壤杆菌 菜心 定殖 磷 钾 |
| 英文关键词: Agrobacterium species Chinese flowering cabbages colonization phosphorus potassium |
| 基金项目:国家自然科学基金项目（41673094）；广东省科技计划项目（2017A010105005） |
| 作者 | 单位 | E-mail | | 吕耀 | 暨南大学环境学院, 广东省环境污染与健康重点实验室, 广州 510632 | | | 王立立 | 暨南大学环境学院, 广东省环境污染与健康重点实验室, 广州 510632 | | | 徐智敏 | 暨南大学环境学院, 广东省环境污染与健康重点实验室, 广州 510632 | | | 李取生 | 暨南大学环境学院, 广东省环境污染与健康重点实验室, 广州 510632 | liqusheng@21cn.com | | 林欣 | 暨南大学环境学院, 广东省环境污染与健康重点实验室, 广州 510632 | | | 周婷 | 暨南大学环境学院, 广东省环境污染与健康重点实验室, 广州 510632 | | | 叶汉杰 | 暨南大学环境学院, 广东省环境污染与健康重点实验室, 广州 510632 | | | 高琼 | 暨南大学环境学院, 广东省环境污染与健康重点实验室, 广州 510632 | |
|
| 摘要点击次数: 2183 |
| 全文下载次数: 1819 |
| 中文摘要: |
| 从农田土中筛选出一株解钾菌T29，结合其形态特征、生理生化特性及16S rDNA序列分析，鉴定其为土壤杆菌属（Agrobacterium sp.）。采用摇瓶试验评估T29对难溶态磷和难溶态钾的活化能力，然后通过趋化、群游、体外及盆栽实验研究不同有机酸和氨基酸诱导下T29在菜心根际的定殖情况。结果表明：摇瓶实验中，T29对难溶态磷和难溶态钾的活化量分别为15.53 mg·L-1和2.78 mg·L-1，分别为对照的1.42倍和1.54倍。趋化和群游实验中，葡萄糖酸诱导下菌株T29的菌落数为3.65×106 cfu·mL-1，菌落直径为2.18 cm，相比对照分别增加130.5%和54.6%。体外实验中，在菜心根际添加了葡萄糖酸后，其根表面的菌落数为2.98×106 cfu·g-1根鲜质量，相比对照增加了29.1%。盆栽实验中，接菌后菜心根际土壤中速效磷和速效钾浓度分别比对照增加了37.5%和52.8%。根部的磷和钾的含量与对照相比没有显著变化（P>0.05），但是茎叶中的磷和钾含量相比对照均显著增大，同时菜心的生物量和株高与对照相比均显著提高（P<0.05）。研究表明，不同的有机酸和氨基酸对T29的吸引能力存在较大差异。其中葡萄糖酸作为T29的一种信号分子，能够较好地吸引T29在菜心根部定殖并使其活化土壤中的难溶态磷和难溶态钾以促进菜心的生长，为减少化肥的施用提供了可能性。 |
| 英文摘要: |
| This bacterial strain, which has potassium-solubilizing ability, was isolated from farmland soils. T29 was identified as Agrobacterium species based on morphological, physiological, and biochemical characteristics and 16S rDNA sequence analysis. Shake flask experiments were used to assess the ability of T29 to mobilize insoluble phosphorus and potassium. Then, the colonization of the rhizosphere by T29, induced by various organic acids and amino acids, was investigated via chemotaxis, swarming, in vitro, and pot experiments. In the shake flask experiment, the mobilized phosphorus and potassium contents were 15.53 mg·L-1 and 2.78 mg·L-1, respectively, which were 1.42 times and 1.54 times those of the control groups. In the chemotaxis and swarming experiments, the number of colonies and the diameter of strain T29 grown in culture medium with gluconic acid were 3.65×106 cfu·mL-1 and 2.18 cm, respectively. In the in vitro experiments, the colony number on the root surface was 2.98×106 cfu·g-1 fresh root weight and was 29.1% higher than that of the control groups. In the pot experiments, compared with the control groups, available phosphorus and potassium in the rhizosphere soil increased by 37.5% and 52.8%, respectively. The phosphorus and potassium concentrations in roots did not change significantly (P>0.05), but those in shoots increased significantly. Compared with the control groups, plant biomass and height increased significantly (P<0.05). These results suggested various organic acids and amino acids influence T29 colonization differently. Gluconic acid preferably strengthened the colonization process of T29 in the rhizosphere of Chinese flowering cabbages, thereby mobilizing more soil phosphorus and potassium and promoting plant growth. Our study provides a possible method for reducing the use of chemical fertilizers. |
| HTML
查看全文
查看/发表评论 下载PDF阅读器 |
|
|
|
