| 史鼎鼎,梁小迪,徐少慧,蒋代华,黄智刚.EDTA与耐性细菌对黑麦草吸收复合污染红壤中铅镉的影响[J].农业环境科学学报,2018,37(8):1634-1641. |
| EDTA与耐性细菌对黑麦草吸收复合污染红壤中铅镉的影响 |
| Effects of EDTA and resistant bacteria on the uptake of Pb and Cd by ryegrass grown in Pb and Cd-contaminated soil |
| 投稿时间：2017-10-05 |
| DOI：10.11654/jaes.2017-1349 |
| 中文关键词: 黑麦草 耐性细菌 EDTA 重金属 联合修复 |
| 英文关键词: ryegrass resistant bacteria EDTA heavy metals joint repair |
| 基金项目:国家自然科学基金项目（41661076）；广西自然科学基金项目（2014GXNAFAA118287） |
|
| 摘要点击次数: 1882 |
| 全文下载次数: 1973 |
| 中文摘要: |
| 为比较耐性细菌与EDTA两种方法强化植物富集重金属效果的优劣，以黑麦草（Lolium perenne L.）为供试植物，利用土培试验和室内分析，比较EDTA与耐性细菌（Rhodococcus baikonurensis，编号为J6）对黑麦草吸收重金属复合污染土壤中铅（Pb）、镉（Cd）的影响。试验采用双因素完全随机区组设计，因素一为Cd、Pb污染浓度，设置6个水平，各个水平的Cd、Pb浓度分别为0、0 mg·kg-1；5、50 mg·kg-1；10、100 mg·kg-1；20、200 mg·kg-1；50、400 mg·kg-1；80、800 mg·kg-1，并依次以代号A1、A2、A3、A4、A5、A6表示。因素二为分别添加EDTA、接种J6菌，以空白为对照。结果表明：在A1、A2条件下，添加J6菌可促进黑麦草生长，提高生物量。接种J6菌对黑麦草吸收Pb、Cd具有促进作用，在不同浓度的重金属处理条件下，接菌后的黑麦草地上部Cd含量呈提高趋势，最高达27%、地下部Pb、Cd含量也分别提高17%~64%、5%~23%；而添加EDTA后的黑麦草仅有地下部Pb含量提高40%（A5）、60%（A6）。接种J6菌可降低根际土的Cd、Pb全量，同时提高有效态Cd、Pb含量。接种J6菌也可提高黑麦草Cd的转运系数。总体而言，接种J6菌可促进黑麦草对重金属复合污染土壤中的Cd、Pb的吸收，其效果优于添加螯合剂EDTA。 |
| 英文摘要: |
| A soil culture test and laboratory analysis was conducted to compare the remediation effect of EDTA and resistant bacteria(strain number J6)on the uptake of Pb and Cd by ryegrass in contaminated soil. A two-factor randomized block design was used, and the first factor was the concentration of Cd and Pb. We set six levels(Cd and Pb levels 0, 0 mg·kg-1; 5, 50 mg·kg-1; 10, 100 mg·kg-1; 20, 200 mg·kg-1; 50, 400 mg·kg-1; and 80, 800 mg·kg-1, respectively), coded A1, A2, A3, A4, A5, and A6. The second factor was different enhancers, which were EDTA, J6 bacteria, and blank. Our results showed that under the conditions of A1 and A2, adding J6 could promote the growth of ryegrass and increase biomass. The inoculation of J6 bacteria could promote the uptake of Pb and Cd in ryegrass. Under different concentrations of heavy metals, the content of Cd in the upper ryegrass was increased after the treatment with bacteria. The highest content was 27%, and the content of Pb and Cd in the underground part increased by 17%~64% and 5%~23%, respectively. After adding the EDTA, the Pb concentration of the plant root increased by 40% and 60% in A5 and A6, respectively. The inoculation of J6 could significantly reduce the total amount of Pb and Cd in rhizosphere soils; meanwhile, J6 could effectively improve the available content of Pb and Cd. Additionally, the inoculation of J6 bacteria could also improve the transport coefficient of Cd in ryegrass. In general, the inoculation of J6 bacteria could promote the uptake of Cd and Pb by ryegrass in soil polluted by heavy metals to a greater extent than did the addition of chelator EDTA. |
| HTML
查看全文
查看/发表评论 下载PDF阅读器 |
|
|
|
