| 李海龙,李香真,聂三安,李云云,郑朝元,王果.水分管理对Cd-Pb-Zn污染土壤有效态及水稻根际细菌群落的影响[J].农业环境科学学报,2018,37(7):1456-1467. |
| 水分管理对Cd-Pb-Zn污染土壤有效态及水稻根际细菌群落的影响 |
| Effects of two flooding methods on bio-available Cd-Pb-Zn contents of contaminated paddy soil and bacteria community in rice rhizosphere |
| 投稿时间：2018-06-01 |
| DOI：10.11654/jaes.2018-0726 |
| 中文关键词: 连续淹水 干湿交替 复合污染 生长时期 根际 细菌群落 |
| 英文关键词: continuous flooding intermittent flooding complex contaminated,growth stage,rhizosphere,bacteria community |
| 基金项目:国家自然科学基金促进海峡两岸科技合作联合基金项目（U1305232） |
| 作者 | 单位 | E-mail | | 李海龙 | 福建农林大学资源与环境学院, 福建省土壤环境健康与调控重点实验室, 福州 350002 | | | 李香真 | 福建农林大学资源与环境学院, 福建省土壤环境健康与调控重点实验室, 福州 350002
中国科学院成都生物研究所环境与应用重点实验室, 环境微生物四川省重点实验室, 成都 610041 | | | 聂三安 | 福建农林大学资源与环境学院, 福建省土壤环境健康与调控重点实验室, 福州 350002 | | | 李云云 | 福建农林大学资源与环境学院, 福建省土壤环境健康与调控重点实验室, 福州 350002 | | | 郑朝元 | 福建农林大学资源与环境学院, 福建省土壤环境健康与调控重点实验室, 福州 350002 | | | 王果 | 福建农林大学资源与环境学院, 福建省土壤环境健康与调控重点实验室, 福州 350002 | 1400619353@qq.com |
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| 中文摘要: |
| 在镉-铅-锌污染土壤上，为了探究水稻根际细菌群落多样性及结构在不同水分条件下的动态变化，采用高通量测序技术，研究了连续淹水（CF）和干湿交替（IF）两种水分模式下，镉-铅-锌复合污染的农田土壤在水稻分蘖期和成熟期根际区细菌群落组成和多样性，以揭示水稻根际区细菌群落在两种水分调控下的响应机制。研究结果表明，水分管理对土壤中Cd、Pb、Zn、Ni有效性和有效N和P含量影响显著。在水稻分蘖期，CF处理Cd、Pb、Zn、Ni有效性显著高于IF处理；到了成熟期，其Cd、Pb、Zn含量显著低于IF土壤。有效N含量在水稻分蘖期，CF处理土壤显著高于IF处理；而在两个生长时期，IF处理的有效P含量均显著高于CF处理土壤。水分管理对细菌群落α多样性影响主要表现在水稻分蘖期，主要是不同水分条件和土壤理化性质共同作用的影响。细菌群落丰度组成在水稻两个生育时期表现均有差异。在水稻分蘖期，淹水处理下的肠杆菌科（Enterobacteriaceae）和莫拉菌科（Moraxellaceae）的相对丰度高于干湿交替处理，其丰度与土壤Zn、Ni和Pb有效量之间呈显著正相关；干湿交替处理Planococcaceae群落相对丰度高于淹水处理，其丰度与有效P之间呈正相关。到了成熟期，在淹水环境下地杆菌科（Geobacteraceae）和粘球菌（Myxococcaceae）的相对丰度高于干湿交替处理，地杆菌科（Geobacteraceae）的丰度与有效P之间呈负相关。在植稻初期，土壤重金属的有效性是影响细菌群落结构的主要因素，随着植稻时间的延长，土壤养分转而成为决定根际细菌群落变动的关键因素。 |
| 英文摘要: |
| In this study, to identified the respond of bacteria community in rice rhizosphere under two water regimes on the Cd-Pb-Zn contaminated paddy soil. Investigating bacteria community component and diversity in rice rhizosphere under continuous flooding(CF) and intermittent flooding(IF) designed, by high-through sequences technology, at tillering and ripening stage of rice, respectively. From results of this study, flooding regimes could significant impact on bio-available N, P, Cd, Pb, Zn, Ni contents in soil. At rice tillering stage, bio-available Cd, Pb, Zn, Ni contents in CF treatment were significant higher than IF treatment, while bio-available Cd, Pb, Zn contents in CF treatment were significant lower than IF treatment. In addition, bio-available N content in CF plots was significant higher than IF plots at tillering stage, but bio-available P content in IF plots was significant higher than CF plots at two growth stages. In addition, the effects of flooding regime on α diversity primary performed at rice tillering stage, which due to reaction of flooding conditions and soil chemical characters. Furthermore, through investigated the change of bacteria abundance and structure, we observed that flooding regime could impact on bacteria structure significantly at two growth stages. At tillering stage, relative abundance of Enterobacteriaceae and Moraxellaceae under CF plots are higher than IF plots, which shown strongly correlation with bio-available Zn, Ni, Pb contents, while abundance of Planococcaceae under IF plots were higher than CF plots, which may due to bioavailable phosphorus contents. When the rice repining stage, abundance of Geobacteraceae and Myxococcaceae under CF plots were higher than IF plots, and Geobacteraceae community shown negative correlation with bioavailable phosphorus contents in soil. In present study, we use molecular biology Techniques to understand the linkage reaction of bacteria community and available ions contents in paddy soil by different flooding regime, and find soil ions contents, affecting by flooding management, would be key factor to impact on bacteria community change. |
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