| 李娟,田保华,刘旦梅,裴雁曦.硫化氢调节谷子幼苗P-ATPase响应镉胁迫[J].农业环境科学学报,2018,37(1):52-57. |
| 硫化氢调节谷子幼苗P-ATPase响应镉胁迫 |
| Hydrogen sulfide regulates cadmium stress resistance through P-ATPases in foxtail millet seedlings |
| 投稿时间：2017-06-27 |
| DOI：10.11654/jaes.2017-0908 |
| 中文关键词: 谷子幼苗 硫化氢 镉胁迫 三磷酸腺苷酶 |
| 英文关键词: foxtail millet seedlings hydrogen sulfide cadmium stress adenosine triphosphate |
| 基金项目:国家自然科学基金面上项目（31671605）；国家自然科学基金青年科学基金项目（31501772）；山西省应用基础研究计划项目（201601D021097） |
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| 摘要点击次数: 2049 |
| 全文下载次数: 2370 |
| 中文摘要: |
| 为了研究H2S对谷子幼苗中ATPases活性的影响及其与金属转运蛋白HMAs基因表达调控的关系，以及H2S对谷子幼苗细胞内Cd离子的解毒作用。在Cd胁迫条件下，对可溶性糖和可溶性蛋白以及ATPase活性进行测定，对谷子HMA家族转运蛋白进行系统进化分析并对H2S对转运蛋白HMAs转录水平的影响进行分析。结果表明，在Cd胁迫条件下，谷子幼苗的可溶性糖和可溶性蛋白含量降低，ATPase活性被抑制，同时HMA家族基因表达量升高；H2S处理可使可溶性糖含量提高的同时增强ATPase的活性，此外HMA的基因表达量也有相应变化。研究表明，硫化氢可以调节谷子幼苗P-ATPase的表达和活性，进而缓解Cd胁迫对谷子幼苗的毒害作用。 |
| 英文摘要: |
| Hydrogen sulfide(H2S), a newly found gasotransmitter, has been shown to play crucial roles in the development of plants, as well as in their resistance to various biotic or abiotic stresses. Cadmium is one of the heavy metals that are highly toxic to the growth of plants. In this study, the relationship among the exogenous H2S, the activity of key ATPases, and the expression levels of heavy metal ATPase(HMA) genes, which encode a class of metal transporters, was analyzed under Cd stress conditions, and the mechanism to relieve Cd toxicity in foxtail millet was eventually determined. We determined the contents of soluble sugar and proteins and the activities of ATPases in the foxtail millet seedlings growing under Cd stress conditions. Additionally, the phylogenetic analysis of HMA proteins and the expression level analysis of the HMA genes were carried out under the exogenous application of H2S and Cd. In the seedlings growing under Cd stress, the contents of soluble sugars and proteins were decreased, the activity of ATPases(Ca2+-ATPase, Mg2+-ATPase, and H2+-ATPase) were inhibited, and the expression levels of HMA genes in the P1B-ATPase family were increased. The H2S treatment could increase the content of soluble sugar and the activities of three kinds of ATPases, upregulate the expression levels of HMA1 and HMA2, and down regulate the expression level of HMA3. These results showed that H2S could regulate the activities of P-ATPases to alleviate Cd stress in foxtail millet seedlings. |
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