| 李维宏,杨宁,魏晓峰,岳秀萍,徐建.一株Cr(Ⅵ)还原菌的筛选鉴定及其还原特性研究[J].农业环境科学学报,2015,34(11):2133-2139. |
| 一株Cr(Ⅵ)还原菌的筛选鉴定及其还原特性研究 |
| Isolation, Identification and Cr(Ⅵ)-Reducing Capacity of a Cr(Ⅵ)-Reducing Strain Enterobacterhormaechei |
| 投稿时间：2015-04-15 |
| DOI：10.11654/jaes.2015.11.014 |
| 中文关键词: 霍氏肠杆菌 Cr(Ⅵ) 还原菌 筛选鉴定 |
| 英文关键词: Enterobacterhormaechei chromium(Ⅵ) reducing bacteria screening and identification |
| 基金项目:土壤重金属污染修复及农田环境安全评价技术体系研究,山西省科技攻关项目(20120311008-1) |
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| 中文摘要: |
| 从山西某铬渣堆场土壤中分离得到一株能还原Cr(Ⅵ)的细菌C2L,通过形态特征、生理生化反应和分子生物学鉴定确定该菌株为霍氏肠杆菌(Enterobacterhormaechei).该菌株能在较宽的pH 值(5~9)和温度(25~45 ℃)范围内还原Cr(Ⅵ),最佳反应条件为pH8和30 ℃.在Cr(Ⅵ)初始浓度为50、100、200、400、800 mg·L-1时,C2L菌株对Cr(Ⅵ)的还原效率分别为99.3%、94.2%、85.6%、82.1%、58.2%.实验结果表明,该菌株在高Cr(Ⅵ)浓度下仍可表现出良好的还原能力,具有独特的还原Cr(Ⅵ)性能,在处理Cr(Ⅵ)污染土壤中存在极大的应用潜力. |
| 英文摘要: |
| Hexavalent chromium Cr(Ⅵ) is a highly toxic and mobile metal species. Reducing Cr(Ⅵ) to Cr(Ⅲ) has been considered to be one of remediation techniques for Cr(Ⅵ)-polluted water or soil. Here one Cr(Ⅵ)-reducing bacterial strain, C2L, was isolated from a Cr(Ⅵ)-polluted soil in Shanxi Province. The strain was identified as Enterobacterhormaechei according to its morphological characteristics, physiological and biochemical reactions, and 16S rDNA sequence analysis. To our knowledge, this is the first report on Cr(Ⅵ) reduction by Enterobacterhormaechei bacterial strain. The reduction of Cr(Ⅵ) occurred over a wide range of pH(5~9) and temperature(25~45 ℃) with the optimum performance at pH 8 and 30 ℃. At the initial Cr(Ⅵ) concentrations of 50 mg·L-1, 100 mg·L-1, 200 mg·L-1, 400 mg·L-1 and 800 mg·L-1, the reduction rates of Cr(Ⅵ) by the C2L strain were 99.3%, 94.2%, 85.6%, 82.1%, and 58.2%, respectively. The strain exhibited Cr(Ⅵ) reduction even at high Cr(Ⅵ) concentrations, suggesting that the strain possessed distinct capability for the Cr(Ⅵ) removal. This strain shows great potential to remediate Cr(Ⅵ)-polluted soils. |
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