| 王丽,韩艳楠,金珊,赵青松,陈寅儿,王春琳.水体Cu2+对三疣梭子蟹主要组织ROS含量和抗氧化能力的影响[J].农业环境科学学报,2015,34(7):1261-1268. |
| 水体Cu2+对三疣梭子蟹主要组织ROS含量和抗氧化能力的影响 |
| Effects of Aqueous Copper on Reactive Oxygen Species Content and Anti-oxidation Capacity of Major Tissues in Portunus trituberculatus |
| 投稿时间：2015-01-27 |
| DOI：10.11654/jaes.2015.07.006 |
| 中文关键词: 三疣梭子蟹 Cu2+ 活性氧 超氧化物歧化酶 总抗氧化能力 |
| 英文关键词: Portunus trituberculatus Cu2+ ROS SOD T-AOC |
| 基金项目:浙江省教育厅项目(Y201224126);宁波市科技创新团队(2011B81003);宁波大学学科项目(XKL11093) |
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| 中文摘要: |
| 为探究水体Cu2+对三疣梭子蟹主要组织氧自由基水平和抗氧化能力的影响,采用生态学单因子梯度试验方法,研究了不同浓度水体Cu2+(0.04、0.4、2、4 mg·L-1)胁迫下三疣梭子蟹鳃、肝胰腺、肌肉组织中的活性氧(ROS)含量、超氧化物歧化酶(SOD)活性和总抗氧化能力(T-AOC)的变化。结果表明:与对照组相比,低浓度水体Cu2+(0.04、0.4 mg·L-1)对三疣梭子蟹鳃、肝胰腺和肌肉组织ROS含量没有显著影响,而高浓度水体Cu2+(2、4 mg·L-1)会导致鳃、肝胰腺和肌肉组织ROS含量显著升高,且升高的幅度与Cu2+浓度成正比。低浓度水体Cu2+(0.04、0.4 mg·L-1)对三疣梭子蟹鳃、肝胰腺和肌肉组织的SOD活性和T-AOC均具有诱导作用,随实验时间的延长,SOD活性和T-AOC呈峰值变化;而高浓度水体Cu2+(2、4 mg·L-1)短时间内对梭子蟹的鳃、肝胰腺、肌肉组织的SOD活性及鳃T-AOC具有诱导作用,但对肝胰腺和肌肉T-AOC却有明显的抑制作用,在实验时间内两种组织T-AOC始终低于对照组,并呈逐渐下降趋势。由此说明低于0.4 mg·L-1的水体Cu2+在三疣梭子蟹抗氧化解毒能力的可控范围,但高于2 mg·L-1的水体Cu2+会对三疣梭子蟹主要组织细胞造成损伤,使之抗氧化解毒能力下降。抗氧化酶和非酶抗氧化物在三疣梭子蟹抵御Cu2+胁迫中共同发挥作用,三种组织SOD和T-AOC表现出明显的时间和剂量效应。 |
| 英文摘要: |
| High levels of copper in water can affect aquacultural production. Here an experiment was designed to examine the responses of reactive oxygen species(ROS), superoxide dismutase(SOD) and total anti-oxidation capacity(T-AOC) in gill, hepatopancreas and muscle of Portunus trituberculatus exposed to different concentrations of Cu2+(0, 0.04, 0.4, 2 and 4 mg·L-1) using single factor gradient method of ecology. The ROS level increased with elevated concentrations of Cu2+(2 mg·L-1 and 4 mg·L-1), but was no significant differences(P>0.05)between 0.04 and 0.4 mg·L-1. Compared with control, SOD and T-AOC activities in gill, hepatopancreas and muscle were induced by low concentrations of Cu2+(0.04 and 0.4 mg·L-1), and displayed an inverted V-shape over the experimental period. Furthermore, the activities of SOD and T-AOC in gill, hepatopancreas and muscle showed obvious relationships between concentration-time effects and time-effect. Under high concentrations of Cu2+(2 and 4 mg·L-1), the activity of SOD in gill, hepatopancreas and muscle and the activity of T-AOC in gill were induced during the initial 24 h, but activity of T-AOC in hepatopancreas and muscle were inhibited at the beginning and decreased markedly over time. The present results suggest that exposure to low(<0.04 mg·L-1) and short-term exposure to high Cu2+ concentrations(>2 mg·L-1) would not cause significant oxidative damages, but enhance the antioxidant defense system. Under extended exposure to high Cu2+, however, oxidative damage was obviously increased, decreasing the antioxidation capacity. Antioxidant enzyme and non-enzymatic antioxidants could jointly play a role in the defense system. |
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