| 王霖,种云霄,余光伟,龙新宪.黑臭底泥硝酸钙原位氧化的温度影响及微生物群落结构全过程分析[J].农业环境科学学报,2015,34(6):1187-1195. |
| 黑臭底泥硝酸钙原位氧化的温度影响及微生物群落结构全过程分析 |
| Temperature Effect on and Microbial Community Structure Dynamics During In-situ Oxidation of Black and Odorous Sediment by Calcium Nitrate |
| 投稿时间：2015-01-07 |
| DOI：10.11654/jaes.2015.06.024 |
| 中文关键词: 黑臭底泥 硝酸钙 温度 微生物群落结构 DGGE |
| 英文关键词: black and odorous sediment calcium nitrate temperature microbial community structure DGGE |
| 基金项目:国家青年基金项目(51108196);广东省自然科学基金项目(S2011040001251) |
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| 中文摘要: |
| 为探明低温的影响, 在室温下(12~22 ℃)模拟研究了投加硝酸钙进行底泥原位修复反硝化除硫的效果, 同时在恒温条件(30 ℃)下研究了硝酸钙投加后、反硝化期间和硝酸盐耗尽后底泥微生物群落结构全过程变化。结果表明, 硝酸钙对底泥硫化物有明显的去除效果, 其中温度是影响硫化物去除的关键因素, 当温度低于15 ℃, 硫化物去除缓慢。DGGE联合克隆测序结果显示投加硝酸钙促进了底泥微生物多样性的增加, 原泥、投加硝酸钙当天、反硝化期间和硝酸盐耗尽后4个时期底泥微生物群落结构存在明显差异。原底泥以兼性硫氧化菌Sulfuricurvum sp.(相似度96%)为主, 投加硝酸钙后, 异养反硝化菌Thermomonas sp.(相似度99%)数量增加;而在硝酸盐耗尽末期, 反硝化菌条带变弱, 而具备丙酸盐氧化功能的Smithella sp.(相似度97%)逐渐成为优势菌群。研究结果可为黑臭底泥硝酸钙原位氧化提供技术参考, 同时从微生物学角度进一步解释了硝酸钙对底泥硫化物和有机物的去除机理。 |
| 英文摘要: |
| Black and odorous river sediment contain large amount of sulfides. Calcium nitrate is an effective agent for in-situ oxidation remediation. A simulated experiment was designed to investigate the effects of low temperatures(12 ℃ to 22 ℃) on sulfur oxidation-denitrification. Changes of microbial community structure in sediments at different stages of oxidation process were also investigated at a constant temperature of 30 ℃ using DGGE combined with clone and sequencing techniques. Results showed that an effective sulfide removal by calcium nitrate occurred during the denitrification process, and the temperature was a key factor. The removal rate of sulfide was very low at temperature lower than 15 ℃。 Calcium nitrate addition increased the microbial diversity in the treated sediment. Significant differences of microbial community structure were observed between day 0(initial untreated sediment), day 1, day 3-14(denitrification process) and day 50(calcium nitrate exhausted) of calcium nitrate addition. Sulfur-oxidizing bacterium Sulfuricurvum sp.(96% similarity) was found to be the main strain in the original sediments, whereas heterotrophic denitrifier Thermomonas sp.(99% similarity) increased with the progress of denitrification. However, the band intensity of Sulfuricurvum sp. and Thermomonas sp. became quite weak when calcium nitrate was exhausted, but Smithella sp.(97% similarity), a propionate oxidizing bacterium, gradually became the dominant species. These findings advance our understanding and application of the calcium nitrate in-situ oxidation technology. |
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