| 昝树婷,周刚,李静,苏楠楠,邵宗圆,杨如意.耐铜细菌及其菌群对纳米CuO的淋滤作用研究[J].农业环境科学学报,2015,34(6):1082-1089. |
| 耐铜细菌及其菌群对纳米CuO的淋滤作用研究 |
| Leaching of CuO Nanoparticles with Copper Tolerant Bacteria and Their Communities |
| 投稿时间：2015-01-20 |
| DOI：10.11654/jaes.2015.06.010 |
| 中文关键词: 重金属 驯化 淋滤 |
| 英文关键词: heavy metal acclimation leaching |
| 基金项目:国家自然科学基金(41001368);安徽高校省级自然科学研究重点项目(KJ2009A026) |
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| 中文摘要: |
| 通过PCR-DGGE和测序等方法分析了铜累积性植物茵陈蒿(Artemisia capillaris Thunb.)根际的细菌群落, 并研究了耐铜细菌对纳米CuO(CuO NPs)的淋滤作用, 试图提高CuO NPs的生物有效性, 以加速修复进程。结果表明, 茵陈蒿根际细菌多样性很低, 主要由气球菌属、寡养单胞菌属和微球菌属组成, 其中气球菌属为优势属。低浓度CuO NPs处理对细菌S31的生长有一定促进作用, 但400 mg·L-1的CuO NPs则会对其产生明显抑制;不同浓度下, 细菌S31对CuO NPs的淋滤作用有明显差异;驯化不仅明显改善了细菌的生长状况, 还进一步增强了CuO NPs的淋滤。通过微生物提高人工纳米材料生物有效性的方法能否实际用于环境修复还有待进一步的试验进行验证。 |
| 英文摘要: |
| Behavior, environmental fate and biotoxicity of manufactured nano materials(MNMs) have received extensive attention in recent years. However, it is unclear whether MNMs-contaminated soils could be remediated by combined plant and rhizospheric microbes. In this study, bacterial community structure associated with copper(Cu) accumulating plant Artemisia capillaris Thunb. was investigated using PCR-DGGE and sequencing techniques. Subsequently, Cu tolerant bacteria were screened using selective culture medium containing 500 mg·L-1 CuSO4. The effect of Cu tolerant bacteria and bacterial communities on leaching of nano CuO(CuO NPs) was then studied. Results showed that bacterial community in the rhizosphere had very low diversity, and mainly consisted of genus of Aerococcus, Stenotrophomonas and Micrococcus, with Aerococcus being the dominant genus. The growth of bacterium S31 was enhanced by low CuO NPs, but suppressed by 400 mg·L-1 CuO NPs. The Cu tolerant bacterium S31 showed a concentration dependent enhancement on leaching of CuO NPs. Acclimation significantly improved the growth of bacterium S31 and increased the leaching of CuO NPs. This study indicates the potential to remediate MNMs-contaminated soil by combined plant and microbes. |
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