| 谢登科,符安,林耀东,秦华明,莫测辉.BDE-209对土壤微生物PLFAs特性的影响[J].农业环境科学学报,2015,34(3):478-484. |
| BDE-209对土壤微生物PLFAs特性的影响 |
| Effect of BDE-209 on Characteristics of Soil Microbial PLFAs |
| 投稿时间：2014-10-18 |
| DOI：10.11654/jaes.2015.03.009 |
| 中文关键词: 十溴联苯醚 土壤微生物 磷脂脂肪酸 土壤环境质量 |
| 英文关键词: decabromodiphenyl ether soil microorganisms phospholipid fatty acids soil environment quality |
| 基金项目:国家自然科学基金项目(41173101);中央高校基本科研业务专项资金项目(21612103) |
| 作者 | 单位 | E-mail | | 谢登科 | 暨南大学环境学院, 广东省高校水土环境毒害性污染物防治与生物修复重点实验室, 广州 510632 | | | 符安 | 暨南大学环境学院, 广东省高校水土环境毒害性污染物防治与生物修复重点实验室, 广州 510632 | | | 林耀东 | 暨南大学环境学院, 广东省高校水土环境毒害性污染物防治与生物修复重点实验室, 广州 510632 | | | 秦华明 | 暨南大学环境学院, 广东省高校水土环境毒害性污染物防治与生物修复重点实验室, 广州 510632 | huamingqin@163.com | | 莫测辉 | 暨南大学环境学院, 广东省高校水土环境毒害性污染物防治与生物修复重点实验室, 广州 510632 | |
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| 中文摘要: |
| 采用室内模拟方法,分析了不同浓度BDE-209处理对土壤微生物磷脂脂肪酸(Phospholipid fatty acids,PLFAs)组成、含量、微生物群落结构和多样性的影响。结果表明,不同浓度BDE-209胁迫下土壤微生物包括内标19:0在内,共检测出20种磷脂脂肪酸,土壤微生物群落PLFAs种类较丰富,PLFAs组成以饱和脂肪酸为主,其中脂肪酸16:0含量最高,单不饱和脂肪酸、多不饱和脂肪酸、支链脂肪酸和环丙烷脂肪酸含量较少。不同浓度BDE-209会改变微生物的组成与含量,与对照(CK)相比,1.0 mg·kg-1处理可将不同种类PLFAs的增加或降低的程度分为3类,10 mg·kg-1和100 mg·kg-1处理的分为4类,增加程度最大的都是细菌。BDE-209对以PLFAs表征的土壤细菌、真菌、放线菌和总微生物量都有显著的影响,且真菌对BDE-209更敏感,细菌对BDE-209耐受更强,其中革兰氏阴性菌对BDE-209污染的响应较敏感,革兰氏阳性细菌更容易存活,而含16:1ω7t的微生物比含16:1ω7c的微生物抗逆性更强。主成分分析发现,对照、1.0、10 mg·kg-1处理和第一主成分呈显著正相关,100 mg·kg-1处理和第二主成分呈显著正相关,其中第一主成分以含16:0、18:0、18:1ω7t、18:2ω6,9的微生物为优势种群,第二主成分以含i17:0、a15:0的微生物为优势种群。PLFAs表征的土壤微生物量和群落结构对BDE-209的响应敏感,可以作为BDE-209污染下指示土壤环境质量变化的有效生物指标。 |
| 英文摘要: |
| Decabromodiphenyl ether(BDE-209) pollution in soils has drawn increasing concerns. However, there is little information available about its impacts on soil microorganisms. A laboratory experiment was designed to examine the composition and contents of microbial phospholipid fatty acids(PLFAs) in soil treated with different concentrations of BDE-209. A total of 20 PLFAs including internal standard 19:0 were detected in soil contaminated by BDE-209, implying relatively abundant microbial community. The compositions of microbial PLFAs were mainly saturated fatty acids, with 16:0 fatty acids being the highest content. The contents of monounsaturated fatty acids, polyunsaturated fatty acids, branched fatty acids and cyclopropane fatty acids were relatively low. Compared with the control, the change of microbial PLFAs was divided into three groups in 1.0 mg·kg-1 BDE-209 treatment, while it was four groups in 10.0 and 100 mg·kg-1 BDE-209 treatments, indicating that BDE-209 concentrations impacted microbial compositions. BDE-209 had significant impacts on the signature PLFAs of soil bacteria, fungi, and actinomycetes, and total microbial biomass. Fungi were most sensitive, but bacteria most tolerant to BDE-209, thus the greatest increases observed in bacteria. The gram-positive bacteria were more likely to survive than the gram-negative bacteria under BDE-209 stress. The microorganisms with 16:1ω7t signature fatty acids were stronger in stress resistance than those with 16:1ω7c signature fatty acids. Principal component analysis showed that the control and the treatments at 1.0 and 10 mg·kg-1 were significantly positively correlated with the first principal component while the treatment at 100 mg·kg-1 was significantly positively correlated with the second principal component. The microorganisms with 16:0, 18:0, 18:1ω7t, 18:2ω6, 9 signature fatty acids were dominant species in the first principal component, and those with i17:0 and a15:0 fatty acids were dominant in the second principal component. These results show that signature PLFAs were sensitive to analyze the responses of soil microbial community to BDE-209 and could be used as an efficient biological indicator of soil environment quality under BDE-209 stress. |
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