| 魏 凤,杨仁斌,蒋 波,傅 强,周 焱.土壤及番茄中嘧肽霉素残留分析方法研究[J].农业环境科学学报,2014,33(2):404-408. |
| 土壤及番茄中嘧肽霉素残留分析方法研究 |
| Analysis of Cytosinpeptidemycin in Soil and Tomato |
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| DOI：10.11654/jaes.2014.02.027 |
| 中文关键词: 嘧肽霉素 分析方法 土壤 番茄 高效液相色谱 |
| 英文关键词: cytosinpeptidemycin analysis method soil tomato HPLC |
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| 中文摘要: |
| 通过探索不同提取方法和提取剂对嘧肽霉素在土壤中的提取效果以及不同萃取剂和层析柱对嘧肽霉素在番茄中的净化效果，建立了高效液相色谱法检测嘧肽霉素在土壤及番茄中的残留分析方法。土壤中的嘧肽霉素采用甲醇-水（3/7，V/V）直接振荡-超声提取；番茄中的嘧肽霉素先采用甲醇匀质提取，提取液经二氯甲烷萃取，再经硅胶层析柱净化，高效液相色谱检测分析。试验结果表明：当嘧肽霉素在土壤、番茄中的添加浓度分别为0.05~1.00 mg·kg-1、0.5~5.00 mg·kg-1时，平均添加回收率分别为87.00%~94.31%、89.96%~107.45%，变异系数分别为1.07%~3.98%、4.66%~9.12%，最低检测浓度分别为0.03 mg·kg-1、0.1 mg·kg-1。对湖南、广州、浙江两年三地的部分样品重复分析可知，该分析方法在土壤中嘧肽霉素的测定变异系数为1.88%~4.56%，在番茄中嘧肽霉素的测定变异系数为3.52%~4.10%。该分析方法均符合农药残留分析方法的检测要求。 |
| 英文摘要: |
| An analytical method of high performance liquid chromatography（HPLC） was developed to detect residues of cytosinpeptidemycin in soil and tomato through examining the effects of different extractants and extracting methods on extraction efficiency of cytosinpeptidemycin in soil and the effects of different extracting solvents and column chromatography on cytosinpeptidemycin extraction from tomato. Cytosinpeptidemycin in soil was extracted by the mixture of methanol and water（3/7, V/V）, and detected by HPLC, while that in tomato was extracted by methanol, and further by methylene-chloride. The extracts were then purified with silica gel column and detected by HPLC. The average recoveries of cytosinpeptidemycin ranged from 87.00% to 94.31% and 89.96% to 107.45%, with the coefficient of variation from 1.07% to 3.98% and 4.66% to 9.12%, for soil at 0.05~1.00 mg·kg-1 and tomato at 0.5~5.00 mg·kg-1, respectively. The minimum detectable concentrations were 0.03 mg·kg-1 for soil and 0.1 mg·kg-1 for tomato. The method developed in the current study meets the requirements of detecting pesticide residue in soil and plant. |
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