文章摘要
王桂萍,郭明志,陈亚华,沈振国,夏 妍.抗铜细菌对难溶性铜的活化及其强化植物修复铜污染土壤[J].农业环境科学学报,2014,33(2):332-338.
抗铜细菌对难溶性铜的活化及其强化植物修复铜污染土壤
Solubilization of Insoluble Copper and Enhancement of Phytoremediation by Copper-resistant Bacteria Inoculated to Copper-contaminated Soil
  
DOI:10.11654/jaes.2014.02.018
中文关键词: 抗铜细菌,溶解作用,联合修复
英文关键词: copper-resistant bacteria  solubilization  plant-microbial remediation
基金项目:
作者单位
王桂萍 南京农业大学生命科学学院 南京 210095 
郭明志 南京农业大学生命科学学院 南京 210095 
陈亚华 南京农业大学生命科学学院 南京 210095 
沈振国 南京农业大学生命科学学院 南京 210095 
夏 妍 南京农业大学生命科学学院 南京 210095 
摘要点击次数: 2009
全文下载次数: 2036
中文摘要:
      研究了2株抗铜细菌F16a和Fw17a在不同培养条件下,对培养基溶液和铜污染土壤中难溶性铜的活化特性,并结合盆栽试验调查其强化植物修复铜污染土壤的效果。结果表明,在液体有氮培养基(含500 mg·L-1 CuCO3)中,菌株F16a能显著提高培养基溶液中铜离子的浓度,Fw17a则能显著降低培养基溶液中铜离子的浓度。培养基溶液中铜浓度增加(或降低)幅度和速率随着F16a(或Fw17a)接种量增加而增大。当F16a和Fw17a分别处于35 ℃和30 ℃培养温度时,其活化或钝化铜的效果最佳。在Cu污染土壤中加入F16a菌悬液能显著提高土壤溶液中的Cu浓度,而Fw17a对污染土壤Cu的钝化效果不明显。盆栽试验结果表明,接入菌株F16a后,能显著提高三叶草和香根草地上部对污染土壤中铜的累积及提取量。
英文摘要:
      This study investigated the effects of two copper(Cu)-resistant bacterial strains, F16a and Fw17a, on insoluble Cu solubilization in different cultures and Cu-contaminated soils, and also evaluated their effects on the phytoremediation of Cu-contaminated soils in a pot experiment. Compared with the control, F16a increased water-soluble Cu concentrations, whereas Fw17a decreased water-soluble Cu in supernatant of N-containing liquid medium with 500 mg·L-1 CuCO3. The magnitude of such change(increase or decrease) in water-soluble Cu concentrations increased with amount of F16a(or Fw17a) inoculums at the beginning. F16a and Fw17a had the greatest effects when exposed to their optimum temperatures. F16a significantly enhanced water-soluble Cu in Cu-contaminated soil, but Fw17a had little effects on water-soluble Cu. Inoculation with F16a significantly increased Cu absorption and accumulation in shoots of Trifolium repensa and Vetiver zizanioides in Cu-contaminated soil.
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