| 杨志国,赵建宁,李 刚,修伟明,章秋艳,常 泓,杨殿林.耐草甘膦转基因大豆对土壤线虫多样性的影响[J].农业环境科学学报,2013,32(11):2199-2205. |
| 耐草甘膦转基因大豆对土壤线虫多样性的影响 |
| Effect of Glyphosate-tolerant Soybean on Diversity of Soil Nematodes |
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| DOI：10.11654/jaes.2013.11.014 |
| 中文关键词: 耐草甘膦大豆 土壤线虫 生态指数 冗余分析 |
| 英文关键词: glyphosate-tolerant soybean soil nematode ecological index redundancy analysis（RDA） |
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| 中文摘要: |
| 为了评价耐草甘膦转基因大豆对土壤线虫的影响，在网室盆栽试验条件下，研究了成熟期耐草甘膦转基因大豆M88根际土壤线虫丰度、营养类群组成和生态学指数以及土壤环境因子和线虫群落之间的相关性。结果表明：试验共分离鉴定出23个土壤线虫属，8个功能群，其中非转基因大豆中黄13（Z13）种植条件下分离鉴定出土壤线虫23属，耐草甘膦转基因大豆M88种植条件下分离鉴定出土壤线虫21属；耐草甘膦转基因大豆M88的土壤线虫平均丰度为（1 143.42±135.04）·100 g-1干土，显著高于（P<0.05）非转基因大豆中黄13[（756.83±141.33）·100 g-1干土]；耐草甘膦转基因大豆M88显著升高了杂食捕食性线虫拟桑尼属（Thorneella）的相对丰度（P<0.01）。虽然非转基因大豆中黄13种植条件下的自由生活线虫成熟度指数MI和结构指数SI显著低于耐草甘膦转基因大豆M88（P<0.05），但是两种大豆种植条件下土壤线虫的Shannon-Wiener多样性指数H′、属丰富度指数GR和营养多样性指数TD均无显著差异，说明耐草甘膦大豆M88对线虫群落结构多样性影响不显著。冗余分析RDA排序图显示：线虫功能组Om4和土壤有机质OM呈显著的正相关，而与硝态氮NN呈显著的负相关。 |
| 英文摘要: |
| Transgenic plants may affect microbial community structure and functions in soil ecosystems. A pot culture experiment was conducted to study the responses of soil nematodes to herbicide-tolerant soybean by analyzing the abundance, trophic group composition, ecological indices of soil nematodes and the relationship between soil environmental factors and soil nematode communities using non-transgenic soybean Z13 as the control. Nematodes in the rhizosphere of herbicide-tolerant soybean M88 and non-transgenic soybean Z13 were separated by the Baermann tray method and identified to genus level using an inverted compound microscope. Twenty-three genera and twenty-one genera were found in the rhizosphere of the Z13 and M88, respectively. The average abundance of nematodes was（1 143.42 ± 135.04） individuals per 100 g dry soil for the M88, whereas （756.83 ± 141.33） individuals per 100 g dry soil for the Z13. They were significantly different（P<0.05）. Compared with the Z13, the M88 significantly increased the relative abundance of Thorneella genus（P<0.01）. Although the maturity index（MI） and structure index（SI） of free-living nematodes were significantly higher in the M88 than in the Z13, but no significant differences were found in Shannon-Wiener diversity index H′, Genus richness Index GR and Trophic diversity Index TD between the M88 and Z13. The redundancy analysis（RDA） diagram showed that the functional guilds Om4 had significant positive correlation with organic matter（OM）, but significant negative correlation with nitrate nitrogen（NN）. These results suggest that herbicide-tolerant soybean M88 has no significant effects on the diversity of soil nematode community. |
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