文章摘要
.镉和苄嘧磺隆复合污染胁迫对水稻的基因应答机理的基因芯片分析[J].农业环境科学学报,2013,32(3):.
镉和苄嘧磺隆复合污染胁迫对水稻的基因应答机理的基因芯片分析
Genome-wide Transcriptional Responses in Rice Roots to the Co-contamination of Cadmium and Bensulfuron-methyl by Microarray
  
DOI:10.11654/jaes.2013.03.003
中文关键词: 水稻  复合污染  基因表达  基因芯片  生物信息学
英文关键词: Oryza sativa L.  co-contamination  gene expression  microarray  bioinformatics
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中文摘要:
      在前期研究的基础上设置镉(Cd,45 μmol·L-1)、苄嘧磺隆(BSM,0.25 μmol·L-1)和镉与苄嘧磺隆复合(++,45 μmol·L-1 Cd + 0.25 μmol·L-1 BSM)污染为试验处理组,在培养基中加入等量的蒸馏水作为对照(CK),以丰美占和粤香占两个不同基因型水稻品种为材料,利用Affymetrix水稻基因芯片,研究了丰美占和粤香占幼苗根系中的基因表达对Cd、BSM及其复合污染胁迫的基因应答情况。基因芯片分析数据表明,经Cd、BSM及其复合胁迫处理的两个水稻品种根系中共发现有2144个基因表达上调(ratio≥2),共发现有2346个基因表达下调(ratio≤0.5)。其中,上调基因主要涉及硫吸收与代谢、抗氧化、植物解毒、泛素引导的蛋白质降解、伴侣蛋白等相关基因。这些基因的高效表达能有效地降低Cd和BSM对水稻植株的毒害作用,进而使复合污染处理下的丰美占幼苗具有较高的对Cd(或BSM)胁迫的耐受性。
英文摘要:
      Using the Affymetrix rice genome chip, it was explored that the gene transcriptional responses in YueXiangzhan and FengMeizhan rice roots to critical concentrations of cadmium(Cd, 45 μmol·L-1), bensulfuron-methyl(BSM, 0.25 μmol·L-1) and their co-contamination (++, 45 μmol·L-1 Cd +0.25 μmol·L-1 BSM) respectively. The results of microarray analysis showed that 2144 genes were significantly up-regulated(ratio≥2), and 2346 genes were down-regulated(ratio≤0.5) in the roots of the rice varieties treated with Cd, BSM and ++. Those highly up-regulated genes were classified into several groups, including sulfur assimilation, detoxification, anti-oxidation, protein ubiquitination and chaperones and so on. It revealed the mechanism for which the treatment of Cd combined with BSM alleviated the toxic effects of Cd or BSM individual treatment on FengMeizhan seedlings.
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